miRNA-496 influences the malignant biological behaviors of cervical cancer HeLa cells and the growth of transplanted tumors in nude mice through mTOR / 中国肿瘤生物治疗杂志

@# [摘 要] 目的：探讨miR-496是否通过靶向mTOR影响宫颈癌HeLa细胞的周期、增殖、迁移、侵袭以及裸鼠移植瘤的生长。方法：选取2020年12月至2021年12月期间于河北医科大学第四医院接受手术的74例宫颈癌患者的肿瘤标本和癌旁组织标本，qPCR、WB法和免疫荧光法检测宫颈癌组织中miR-496和mTOR在mRNA和蛋白水平的表达。利用TargetScan数据库预测miR-496的靶基因并用双荧光素酶报告基因实验进行验证。将HeLa细胞按转染物不同分为对照组、miR-496 mimic组和miR-496 mimic+pMIR-mTOR组，CCK-8法、流式细胞术和Transwell实验分别检测miR-496和mTOR对HeLa细胞增殖、周期、迁移和侵袭的影响。将对照组、miR-496 mimic组HeLa细胞接种至BALB/c裸鼠皮下构建宫颈癌裸鼠移植瘤模型，21 d后处死小鼠，剥离小鼠肿瘤组织并称取瘤质量，免疫荧光法和WB法检测miR-496过表达对移植瘤组织中mTOR和Ki67表达的影响。结果：在宫颈癌组织中，miR-496呈低表达，而mTOR mRNA和蛋白呈高表达。miR-496能够靶向结合mTOR mRNA的3’-UTR。与对照组和miR-496 mimic+pMIR-mTOR组相比，miR-496 mimic组HeLa细胞的miR-496水平和S期细胞比例均显著升高，而增殖水平、迁移和侵袭细胞数均显著降低（均P<0.01）。成功构建宫颈癌裸鼠移植瘤模型，接种21 d后，miR-496 mimic组移植瘤质量、移植瘤组织中mTOR和Ki67的表达水平均显著低于对照组（均P<0.01）。结论：miR-496在宫颈癌组织中呈低表达，miR-496过表达可通过靶向调控mTOR抑制HeLa细胞的恶性生物学行为和裸鼠移植瘤的生长。

miR-425-5p suppresses tumorigenesis and DDP resistance in human-prostate cancer by targeting GSK3 β and inactivating the Wnt/β-catenin signaling pathway

In previous studies, we found interferon-a (IFN-a) could reduce protein levels of p11, 5-hydroxytryptamine receptor 1b(5-HT1b) and 5-hydroxytryptamine receptor 4 (5-HT4), but does not influence their messenger RNA levels in SH-sy5ycells. Thus, we investigated the post-transcriptional modulation of these molecules by IFN-a. SH-sy5y cells were treatedwith IFN-a, NH4Cl or MG132 alone or in combination, and then the protein levels of p11, 5-HT1b and 5-HT4 wereanalyzed by western blots. The regulatory effects of p11 on 5-HT1b and 5-HT4 were also determined in p11 knock-downcells. NH4Cl but not MG132 could reverse the protein level of p11 in IFN-a-treated SH-sy5y cells. MG132 could recoverthe protein levels of 5-HT1b and 5-HT4 in p11 knock-down cells. The down-regulation effects of IFN-a on p11, 5-HT1band 5-HT4 were associated with the lysosome and ubiquitin–proteasome-mediated pathways. p11 was identified as a potentregulator to modulate the ubiquitination of 5-HT1b and 5-HT4. Therefore, it could be potential target therapies in IFN-ainduced depression.

Three dimensional measurement study of the correlation between the mandibular Bonwill-Hawley arch form and base bone arch shape in ClassⅠmalocclusion patients / 口腔疾病防治

Objective@#To investigate the correlation between mandibular Bonwill-Hawley arch and base bone archshape. @*Methods@#CBCT and Mimics10.01 software were used to locate and measure the mandibular arch of 32 patients with type Ⅰ malocclusion. The individualized Bonwill-Hawley arch was drawn and the length and width of the arch and base arch were measured, and the data were compared and analyzed by SPSS 16.0 software. @*Results @# Pearson correlation analysis results showed significant correlation between Bonwill-Hawley arch and base bone arch in both length and width (r>0.5). Compared with base bone arch, anterior (t=0.81, P=0.25) and medium (t=0.69, P=0.50)Bonwill-Hawley arch showed no statistical difference in both length and width (P>0.05), while posterior Bonwill-Hawley arch showed significant statistical difference in length (t=2.21, P=0.03) and width (t=2.42, P=0.02). @*Conclusion @#There was significant correlation between Bonwill-Hawley arch and base bone arch in both length and width.

An outbreak of imported dengue fever from Myanmar to the border of China, with its viral molecular epidemiological features / 中华流行病学杂志

<p><b>OBJECTIVE</b>To understand the epidemiologic characteristics of dengue fever, imported from Myanmar to the border of Yunnan province, China. Viral molecular epidemiologic features were also studied.</p><p><b>METHODS</b>Questionnaires were used on each diagnosed, suspected dengue fever, case or unknown cases with fever when coming from Myanmar entering the port and hospitals in Ruili city of Yunnan province. Serum samples of these patients were collected to detect IgM antibody against dengue virus and RT-PCR assay. Homology and phylogenetic tree based on the whole nucleotide sequence of PrM-C and NS5 gene of dengue virus were further analyzed.</p><p><b>RESULTS</b>A total of 103 sera were collected from patients at acute stage in Ruili city in July to November 2008. Among them, 49 cases were confirmed for dengue fever according to IgM and nucleic acid testings. Except one, other 48 cases were all imported into Ruili, from Myanmar. Of those, 18 patients were residents from Mujie city of Myanmar and hospitalized in Ruili and the rest 30 patients were Chinese citizens who had finished business and returned from Myanmar. Two isolates of serum samples from the imported cases were identified and both homology and phylogenetic analysis were performed, using the nucleotide sequences of PrM and NS5 genes. They were divided into dengue type 1 (RLB61) and dengue type 3 (RLC31) and were closer to the dengue virus strains isolated from Southeast Asia countries.</p><p><b>CONCLUSION</b>It is confirmed that an epidemic of dengue fever which was imported from Myanmar to Ruili city of Yunnan province, China. Evidence also showed that both type I and III epidemic strains of dengue virus did exist in Mujie city of Myanmar in 2008.</p>

Clinical study of 99Tc-MDP in the treatment of multiple bone metastases cancer / 中国基层医药

Objective To observe the effect of 99Tc-MDP in the treatment of metastasis bone disease pain with malignancies,and the influence of 99Tc-MDP to human body's Hb,WBC,PLT,serum calcium,and PS grade.Methods 99Tc-MDP was used for the treatment of bone metastases in 78 cases with malignancies:established the intravenous access,mairdined with the 99Tc-MDP,200mg,the diluted physiological brine,and in tWO hours,once a day for five days.After the rest of twenty one to twenty-eight days,the second cycle Was repeated,and each patient at least three cycles.Results 99Tc-MDP had no influence to the Hb,WBC and PLT in pre-treatment and post-treatment,but had obvious effeet on the serum calcium,the hypocalcaemia reduced obviously(P＜0.01).There was significant difference in ameliorating ache during the periods of pretreatment and post-treatment(P＜0.05).However,there was no marked improvement in PS,and no statistic difference(P＞0.05).Conclusion 99Tc-MDP has deftnite curative effect in the treatment of metastasis bone cancer,can ameliorate the ache induced by it,and can reduce the hypoealcaemia.There is no marrow inhibition and enteron reaction in the application.

Glutathione S-transferases null genotype in acute myeloid leukemia

The glutathione S-transferase [GST] family of metabolising enzymes plays an important role in the detoxification of mutagens and carcinogens. The expression of many of these cancer susceptibility enzymes is genetically polymorphic. An increased frequency of GST-null genotypes has been associated with several malignancies. To investigate the rate of GSTT1 and GSTM1 null genotypes in AML patients and to determine its importance in prognosis of the disease. DNA was extracted by phenol/chloroform method from peripheral blood or bone marrow of 180 white Caucasian patients. A multiplex PCR method was used simultaneously to amplify regions of GSTM1, GSTT1, and beta-globin genes in genomic DNA. The survival curves were analyzed by the Kaplan-Meier method and compared by the log-rank test [Mantel-Cox] using the SPSS software program. Of the total of 180 patients, 23 cases [12.8%] showed null genotypes in both genes, while in 52 patients [28.9%] both genes were wild-types. GSTM1 null-GSTT1 wild-type was detected in 91 patients [50.6%] and GSTM1 wild-type-GSTT1 null genotype was detected in 14 patients [7.8%]. These rates are within the upper limit of the rates detected in the normal European population. There was no significant difference in the overall survival and in disease free survival between different groups. These observations suggest that the inherited absence of the GSTT1 and GSTM1 carcinogen detoxification pathway may be related to carcinogenesis but it is not an important determinant of prognosis in AML

Measurement of Replicative Senescence in Long Term Cultured Human Keratinocytes / 대한해부학회지

The keratinocyte culture has been used for the reconstruction of artificial skin or as in vitro skin model. For these purpose, keratinocytes should be cultured for long time (usually 10 cumulative population doublings) and it is important to evaluate the state of replicative senescence or cell senescence during this time. This study was undertaken to investigate senescence associated beta-galactosidase (SA-beta-gal) activity for the senescent cell and keratin 19 immunohistochemistry for the skin stem cell in keratinocytes on ten times serial subculture (referred to 10 cumulative population doublings). Keratinocytes were isolated from foreskin of a 2 day old child, and cultured in KSFM and then in DMEM/F-12. In both keratinocytes cultured in KSFM and DMEM/F-12, lysosomal beta-galactosidase activity, which is detected at pH 4.0, was present in all cells regardless of their replicative age. In contrast, SA-beta-gal activity, which is detected at pH 6.0, was present in a few cells (from 0.1% to 3%) during whole subcultures. These data suggest that most of keratinocytes did not undergo replicative senescence in this culture. Furthermore, in keratin 19 skin stem cell staining, a lot of keratinocytes (13.8%) showed strong positive reaction on the 10th subculture. Together with the results of beta-galactosidase activity, the persistence of high proportion of keratin 19 positive skin stem cells implies further increment of keratinocyte populations by continued subcultures.